NASC Stock Detail Page
NASC ID: N849856
Name: WiscDsLox255G12
ABRC stock number: CS849856
Description: Sequence tagged T-DNA insertion line, generated by vacuum infiltration of Columbia (Col) plants with Agrobacterium tumefaciens vector WiscDs-Lox; Basta herbicide was employed for selection of plants carrying a T-DNA; each T1 transformant has been maintained individually. This line can be utilized as in Ds Launchpad experiments; the T-DNA includes a Ds transposon/launchpad construction so that transposition can be induced by crossing to an Ac line; selection of resulting transposants is by Basta and hygromycin; LoxP sites are also present so that regions between transposed Ds elements and the empty donor site can be subsequently removed by crossing to a Cre line. Associated Polymorphisms: Wiscseq_DsLox255G12.0 : Genbank Annotation 2015-02-08 - This genomic-terminal sequence of a TDNA insertion region lies within the Promoter 500bps of AT1G30135.1 (chr 1 pos 10596331 on the TAIR10). The insertion site is at the 3' end of the sequence mapping. Genbank Acc. KG796846. Wiscseq_DsLox255G12.1 : Genbank Annotation 2015-02-08 - This genomic-terminal sequence of a TDNA insertion region lies within the CDS of AT1G15300 (chr 1 pos 5265753 on the TAIR10). The insertion site is at the 3' end of the sequence mapping. Genbank ID: KG796845. Wiscseq_DsLox255G12.2 : Genbank Annotation 2015-02-08 - This genomic-terminal sequence of a TDNA insertion region lies within the Promoter 500bps of AT3G11510.1 (at chr 3 pos 3625093 (C/3625093-3625177) on the TAIR10). The sequences' 1-85 bps mapped on the genome, 85-100 bps onto the T-DNA. Pools P 0, R 8, C 2 N 7. The insertion site is at the 3' end of the sequence mapping. Genbank Acc. KO452399. Wiscseq_DsLox255G12.3 : Genbank Annotation 2015-02-08 - This genomic-terminal sequence of a TDNA insertion region lies within the Promoter 500bps of AT5G18190.1 (chr 5 pos 6014945 on the TAIR10). The insertion site is at the 3' end of the sequence mapping. Genbank Acc. KG796847.
Donation Date: 2004-12-01
Donated by: | University of Wisconsin, Madison Patrick Krysan University of Wisconsin, Madison Richard Amasino University of Wisconsin, Madison Michael Sussman |
Donor Number: WiscDsLox255G12
Part of : Wisconsin DsLox T-DNA lines
Stock type: individual line
Material type: seed
Status | Price (£) |
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Available | £11.00 |
Germplasm Info
Mutagen: T-DNA Insertion
Background: Col-2
Transformation method: Agrobacterium
Associated Polymorphisms
When available, select a locus to display it at the AIP or view the EMBL record at the EBI.
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